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Screening for Barley yellow dwarf virus-Resistant Barley Genotypes by Assessment of Virus Content in Inoculated Seedlings

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F06%3A250" target="_blank" >RIV/00027006:_____/06:250 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Screening for Barley yellow dwarf virus-Resistant Barley Genotypes by Assessment of Virus Content in Inoculated Seedlings

  • Original language description

    The content of Barley yellow dwarf virus (BYDV) in roots and leaves of barley seedling plants differing in their level of resistance was assessed by quantitative ELISA 1?42 days after inoculation with the strain of BYDV (PAV). High virus accumulation inroots and low concentration in leaves was characteristic of the period 9?15 days after inoculation. In leaves, the differences in virus content between resistant and susceptible genotypes became significant after 15 days and resistance to virus accumulation was better expressed 30?39 days after inoculation. Roots of resistant materials exhibited evident retardation of virus accumulation and the greatest difference in virus content between resistant and susceptible plants was detected 9 days after inoculation. By these criteria, the selected winter and spring barley cultivars and lines (in total 44 materials) fell in to five groups according to field reactions and the presence or absence of the Yd2 resistance gene. There were highly sign

  • Czech name

    Výběr genotypů ječmene odolných ke žluté zakrslosti ječmene pomocí hodnocení obsahu viru v infikovaných klíčních rostlinách

  • Czech description

    Obsah viru žluté zakrslosti ječmene (BYDV) v kořenech a listech klíčních rostlin ječmene s odlišnou úrovní rezistence byl hodnocen pomocí kvantitativní ELISA metody 1-42 dní po infekci BYDV kmenem PAV. Vysoký obsah viru v kořenech a nízký v listech byl charakteristický pro období od 9-15 dní po infekci. V listech se rozdíly mezi náchylnými a rezistentními rostlinami staly významnými 15 dní po infekci a rezistence k akumulaci viru se nejlépe projevovala 30-39 dní po infekci. Kořeny rezistentních rostlinvykazovaly zpoždění v akumulaci viru a největší rozdíly v obsahu viru byly detekovány 9 dní po inokulaci. Dle popisované metody je možno rozlišit rezistentní a mírně rezistentní genotypy ( s genem Yd2 i bez tohoto genu) od náchylných genotypů. Šlechtitelské využití této metody je možné.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GE - Plant cultivation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA521%2F03%2F0137" target="_blank" >GA521/03/0137: Improvement of genetic resistance to abiotic stresses by selection for resistance to BYDV in barley</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Phytopathology

  • ISSN

    0931-1785

  • e-ISSN

  • Volume of the periodical

    154

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    7

  • Pages from-to

    336-342

  • UT code for WoS article

  • EID of the result in the Scopus database