In vitro induction of polyploidy in yacon (Smallanthus sonchifolius)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F09%3A00000767" target="_blank" >RIV/00027006:_____/09:00000767 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41610/09:28955 RIV/60109807:_____/09:0R000268
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
In vitro induction of polyploidy in yacon (Smallanthus sonchifolius)
Original language description
In vitro chromosome doubling was induced in octoploid (2n = 58) yacon using oryzalin and colchicine as mitotic spindle inhibitors. The resulting ploidy level was determined by chromosome counting and flow cytometry. The greatest proportions of hexadecaploid plants (1.6%) were obtained after 48 h of 25 lM oryzalin treatment. With the colchicine treatment, only 0.42% hexadecaploid plants were detected and their survival rate was significantly lower in comparison with the oryzalin treatment. In hexadecaploid yacon, significantly higher levels of saccharides were detected (FOS 13.9 g/100 g FM, fructose 4.6 g/100 g FM and glucose 2.1 g/100 g FM) compared to the octoploid control (FOS 5.3 g/100 g FM, fructose 2.9 g/100 g FM and glucose 1.0 g/100 g FM. Theseresults indicate that in vitro treatment of nodal segments with oryzalin solution could be an effective procedure for chromosome doubling and the polyploidy breeding can help to increase the FOS content in the tuberous roots.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GE - Plant cultivation
OECD FORD branch
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Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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