Protoplast culture and fusion between Brassica carinata and Brassica napus

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F09%3A00000768" target="_blank" >RIV/00027006:_____/09:00000768 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Protoplast culture and fusion between Brassica carinata and Brassica napus
Original language description
Attempts were made to select in vitro responsible genotypes and to fuse the isolated protoplasts of Brassica carinata and Brassica napus breeding lines, obtained from our previous experiments. Combination of three different PEG concentrations and two different treatment durations (15 and 20 min.) were tested. Our experiments identified several genotypes with satisfactory regeneration ability of calli from protoplast cultures. Proper combinations of concentration and treatment time of PEG determined protoplast fusion frequency between genotypes used. Although the 30% PEG solution was evaluated to be the best concentration, large amount of multifusants, unwanted in practical applications, was detected especially in Petri dishes with longer PEG treatment.In general, 25% PEG combined with 20 minutes treatment duration produced satisfactory fusion frequency and good rate of viability was obtained as well.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GE - Plant cultivation
OECD FORD branch
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Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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