Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F09%3A00000920" target="_blank" >RIV/00027006:_____/09:00000920 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic

Original language description

One-step RT-PCR followed by a RFLP assay was developed for the typing of Barley yellow dwarf virus (BYDV) member of Luteovirus genera. A partial coat protein (CP) gene sequencing was carried out and confirmed the RFLP analysis. Both sequencing and RFLP analysis identified the presence of 3 BYDV-PAV (including the lab isolate Blatno85), 10 BYDV-MAV and 10 BYDV-PAS isolates among the field samples. These methods revealed that PAS and MAV are more dominant than PAV in the Czech Republic.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/QH81269" target="_blank" >QH81269: Diagnostic methods and control measures of viruses causing dwarf diseases and their vectors in cereal crops.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cereal Research Communications

ISSN

0133-3720

e-ISSN

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Volume of the periodical

37

Issue of the periodical within the volume

4

Country of publishing house

HU - HUNGARY

Number of pages

10

Pages from-to

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UT code for WoS article

000273068800007

EID of the result in the Scopus database

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