Comparison of specificity and sensitivity of immunochemical and molecular techniques for determination of Clavibacter michiganensis subsp. michiganensis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F10%3A00001266" target="_blank" >RIV/00027006:_____/10:00001266 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/10:00346909

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of specificity and sensitivity of immunochemical and molecular techniques for determination of Clavibacter michiganensis subsp. michiganensis

Original language description

Detection of Clavibacter michiganensis subsp. michiganensis (Cmm), causing bacterial canker of tomato, was verified by means of PTA-ELISA and IFAS with PAbs of Neogen Europe Ltd. (UK), and with published and also laboratory-generated PCR primers from theCmm tomatinase gene. The technique's specificity was tested with 15 plant-pathogenic and 4 common, saprophytic bacteria. Molecular methods were optimised by verification of annealing temperatures and times for both primers. After this optimization stepboth primer pairs produced positive reaction only with Cmm.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/QH71229" target="_blank" >QH71229: Diagnostics and methods of protection against quarantine and other economically important pathogens of fruit and leafy vegetables</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

55

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

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UT code for WoS article

000278469400007

EID of the result in the Scopus database

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