Comparative analyses of proteolytic activities in seven species of synanthropic acaridid mites
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F10%3A00001292" target="_blank" >RIV/00027006:_____/10:00001292 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Comparative analyses of proteolytic activities in seven species of synanthropic acaridid mites
Original language description
Microplate assays with 96 wells were optimized to screen proteolytic activities in mite homogenates. Whole-mite extracts of seven species of mites exhibited non-specific proteolytic activity in buffers from pH 2 to 12, and three peaks of highest activityat pH 3, 5-6, and 10 were distinguished. The reducing agent Tris(2-carboxyethyl) phosphine hydrochloride decreased general proteolytic activity on azocasein at pH 5 and 6. The results obtained on two non-specific substrates, azocasein and azoalbumin, showed highly different ranks of the species at pH 5 and 6. Evidence of the presence of proteolytic activities on all tested substrates and in all the tested mite homogenates suggests that the proteolytic activities may contribute to allergenicity. Poor orundetected hydrolytic activities of mite extracts toward substrates for keratin and collagen at digestive pH underline the importance of ecological interactions between mites and microorganisms in the utilization of such substrates.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Archives of Insect Biochemistry and Physiology
ISSN
0739-4462
e-ISSN
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Volume of the periodical
75
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
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UT code for WoS article
000283778200004
EID of the result in the Scopus database
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