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Comparative analyses of proteolytic activities in seven species of synanthropic acaridid mites

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F10%3A00001292" target="_blank" >RIV/00027006:_____/10:00001292 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparative analyses of proteolytic activities in seven species of synanthropic acaridid mites

  • Original language description

    Microplate assays with 96 wells were optimized to screen proteolytic activities in mite homogenates. Whole-mite extracts of seven species of mites exhibited non-specific proteolytic activity in buffers from pH 2 to 12, and three peaks of highest activityat pH 3, 5-6, and 10 were distinguished. The reducing agent Tris(2-carboxyethyl) phosphine hydrochloride decreased general proteolytic activity on azocasein at pH 5 and 6. The results obtained on two non-specific substrates, azocasein and azoalbumin, showed highly different ranks of the species at pH 5 and 6. Evidence of the presence of proteolytic activities on all tested substrates and in all the tested mite homogenates suggests that the proteolytic activities may contribute to allergenicity. Poor orundetected hydrolytic activities of mite extracts toward substrates for keratin and collagen at digestive pH underline the importance of ecological interactions between mites and microorganisms in the utilization of such substrates.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2010

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Archives of Insect Biochemistry and Physiology

  • ISSN

    0739-4462

  • e-ISSN

  • Volume of the periodical

    75

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    20

  • Pages from-to

  • UT code for WoS article

    000283778200004

  • EID of the result in the Scopus database