Progress in the Czech Hop Germplasm Cryoconservation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F11%3A00002051" target="_blank" >RIV/00027006:_____/11:00002051 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Progress in the Czech Hop Germplasm Cryoconservation
Original language description
In this study the applicability of a new cryopreservation method for conservation of hop germplasm was evaluated. 35 cultivars of Humulus lupulus and one clone of Humulus japonicus were introduced into in vitro conditions, evaluated on viral infection, cryopreserved and recovered for tests of genotype stability. Altogether 72 independent experiments were performed for verification of the cryopreservation method. The average explant post-thaw recovery of all evaluated accessions was 38 % whereas 72 % accessions had post-thaw recovery higher than 30 %. The results guaranteed successful recovery of all cryopreserved cultivars. Tests of genotype stability by SSR and STS analyses did not detect any changes due to cryopreservation.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Project
<a href="/en/project/QF3039" target="_blank" >QF3039: Establishing of cryobank of vegetative shoot tips of potato and hop.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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