Molecular characterization and diagnostics of stubby root and virus vector nematodes of the family Trichodoridae (Nematoda: Triplonchida) using ribosomal RNA genes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F12%3A00002359" target="_blank" >RIV/00027006:_____/12:00002359 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/j.1365-3059.2012.02598.x" target="_blank" >http://dx.doi.org/10.1111/j.1365-3059.2012.02598.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1365-3059.2012.02598.x" target="_blank" >10.1111/j.1365-3059.2012.02598.x</a>

Result language
angličtina
Original language name
Molecular characterization and diagnostics of stubby root and virus vector nematodes of the family Trichodoridae (Nematoda: Triplonchida) using ribosomal RNA genes
Original language description
Plant parasitic nematodes of the family Trichodoridae cause substantial yield losses in many agricultural crops. Rapid and accurate identification of trichodorids to the species level is critical for selection of appropriate measures for control. This study analysed 99 sequences of the D2?D3 expansion segments of the 28S rRNA gene and 131 sequences of the 18S rRNA gene from the stubby nematodes belonging to the genera Nanidorus, Paratrichodorus and Trichodorus. Species delimiting was based on the integration of morphological identification, which is not provided in the present article, and molecular based phylogenetic inference and sequence analysis. Twenty-two valid species and several species complexes were identified among nematodes included in theanalysis. PCR-RFLPs of the partial 18S rDNA and the D2?D3 expansion segments of the 28S rDNA were tested and proposed for identification of these nematodes. Gel PCR-RFLP profiles and tables with restriction fragment lengths for several di
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
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Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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