PCR Detection of the 14.5 Antibacterial NlpC/P60-Like Dermatophagoides pteronyssinus Protein in Dermatophagoides farinae (Acari: Pyroglyphidae)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F13%3A00002592" target="_blank" >RIV/00027006:_____/13:00002592 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1603/ME13027" target="_blank" >http://dx.doi.org/10.1603/ME13027</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1603/ME13027" target="_blank" >10.1603/ME13027</a>
Alternative languages
Result language
angličtina
Original language name
PCR Detection of the 14.5 Antibacterial NlpC/P60-Like Dermatophagoides pteronyssinus Protein in Dermatophagoides farinae (Acari: Pyroglyphidae)
Original language description
House dust mites produce antibacterial proteins suppressing bacterial growth. The 14.5-kDa bacteriolytic protein (UniProtKB Q8MWR6) has been known in Dermatophagoides pteronyssinus Trouessart. We have applied polymerase chain reaction and reverse transcription-PCR to detect a homologous gene sequence coding for a Q8MWR6-related protein in Dermatophagoides farinae (Hughes) using genomic DNA and total RNA, respectively. The resulting PCR product of expected size, 243 bp, was obtained from both Dermatophagoides spp., while no amplification was achieved from stored product mite samples. Sequence of the gene fragment from D. farinae showed 83% similarity to the previously described one in D. pteronyssinus. Successful amplification of the expected product from cDNA generated with oligo-dT primer implies that the NlpC/P60-like protein in Dermatophagoides mites is of eukaryotic or mite origin.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EG - Zoology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Medical Entomology
ISSN
0022-2585
e-ISSN
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Volume of the periodical
50
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
3
Pages from-to
931-933
UT code for WoS article
000321897300033
EID of the result in the Scopus database
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