Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F14%3A00002888" target="_blank" >RIV/00027006:_____/14:00002888 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/14:43898073

Result on the web

<a href="http://dx.doi.org/10.1002/jssc.201301292" target="_blank" >http://dx.doi.org/10.1002/jssc.201301292</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201301292" target="_blank" >10.1002/jssc.201301292</a>

Result language

angličtina

Original language name

Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley

Original language description

The tests were conducted in field conditions at the Crop Research Institute in Prague?Ruzyně. Each barley genotype was sown in a 1 m2 plot at a sowing rate of 450 seeds/m. Within the first set of experiments the following barley varieties were examined:Aktiv, Gladys, Henrike, Lilly, Radegast, Sebastian, Signora, Sladar, and Tolar. Each variety was grown and tested in artificially infected and control variants. Artificially infected samples were inoculated at the beginning of heading using an isolate mixture from F. culmorum isolates.Within the first phase of this methodological study, we focused on the sample preparation for the analysis of the broadest possible spectrum of metabolites/small molecules isolated from the barley tissue. During the courseof our experiments, 1 g of barley grain samples (a pool of control and infected barley of variety Sebastian) was homogenized using an Ultraturrax with 10 mL of different solvents or mixtures of solvents with decreasing polarity. Specific

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

<a href="/en/project/QI111B044" target="_blank" >QI111B044: Comprehensive strategy for decreasing a negative impact of Fusarium spp. toxicogenic fungi infection in cereals and their derivated products.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF SEPARATION SCIENCE

ISSN

1615-9306

e-ISSN

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Volume of the periodical

37

Issue of the periodical within the volume

8

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

912-919

UT code for WoS article

000334059900003

EID of the result in the Scopus database

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