Two-dimensional gel proteomic analysis of Dermatophagoides farinae feces

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F15%3A00003047" target="_blank" >RIV/00027006:_____/15:00003047 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10493-014-9848-1" target="_blank" >http://dx.doi.org/10.1007/s10493-014-9848-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10493-014-9848-1" target="_blank" >10.1007/s10493-014-9848-1</a>

Result language
angličtina
Original language name
Two-dimensional gel proteomic analysis of Dermatophagoides farinae feces
Original language description
In this study, we prepared and using proteomic methods analyzed a D. farinae fecal extract for the first time. This study contains in-depth proteomic mapping of the allergenic isoforms from the D. farinae fecal extract. Despite extensive analysis, MALDITOF/TOF spectrometry showed that only six proteins/allergens, Der f1, Der f2, Der f3, Der f6, Der f15 and ferritin, originated from D. farinae. No other analyzed proteins were exactly assigned to Dermatophagoides or to similar invertebrate species by sequence similarity. Isoforms of the allergens Der f1, Der f3 and Der f15 were identified in more MWs indicating the presence of zymogens and active-enzyme forms. The identified fecal allergens accumulate in the environment during the life of the mite and represent quantitatively greater amounts of mite immunogens than those that were missed in the 2D-E. The results contribute to our understanding of D. farinae digestive physiology with regard to the enzymes/proteins present in the feces.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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