Prevalence of pathogenic bacteria in Ixodes ricinus ticks in Central Bohemia

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003377" target="_blank" >RIV/00027006:_____/16:00003377 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s10493-015-9988-y" target="_blank" >http://dx.doi.org/10.1007/s10493-015-9988-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10493-015-9988-y" target="_blank" >10.1007/s10493-015-9988-y</a>

Result language

angličtina

Original language name

Prevalence of pathogenic bacteria in Ixodes ricinus ticks in Central Bohemia

Original language description

Bacteria associated with the tick Ixodes ricinus were assessed in specimens unattached or attached to the skin of cats, dogs and humans, collected in the Czech Republic. The bacteria were detected by PCR in 97 of 142 pooled samples including 204 ticks, i.e. 1–7 ticks per sample, collected at the same time from one host. A fragment of the bacterial 16S rRNA gene was amplified, cloned and sequenced from 32 randomly selected samples. The most frequent sequences were those related to Candidatus Midichloria midichlori (71 % of cloned sequences), followed by Diplorickettsia (13 %), Spiroplasma (3 %), Rickettsia (3 %), Pasteurella (3 %), Morganella (3 %), Pseudomonas (2 %), Bacillus (1 %), Methylobacterium (1 %) and Phyllobacterium (1 %). The phylogenetic analysis of Spiroplasma 16S rRNA gene sequences showed two groups related to Spiroplasma eriocheiris and Spiroplasma melliferum, respectively. Using group-specific primers, the following potentially pathogenic bacteria were detected: Borellia (in 20 % of the 142 samples), Rickettsia (12 %), Spiroplasma (5 %), Diplorickettsia (5 %) and Anaplasma (2 %). In total, 68 % of I. ricinus samples (97/142) contained detectable bacteria and 13 % contained two or more putative pathogenic groups. The prevalence of tick-borne bacteria was similar to the observations in other European countries.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EG - Zoology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Experimental and Applied Acarology

ISSN

0168-8162

e-ISSN

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Volume of the periodical

68

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

127-137

UT code for WoS article

000366639200010

EID of the result in the Scopus database

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