In vivo and in vitro effectiveness of Azadirachta indica-synthesized silver nanocrystals against Plasmodium berghei and Plasmodium falciparum, and their potential against malaria mosquitoes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003653" target="_blank" >RIV/00027006:_____/16:00003653 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.rvsc.2016.03.001" target="_blank" >http://dx.doi.org/10.1016/j.rvsc.2016.03.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.rvsc.2016.03.001" target="_blank" >10.1016/j.rvsc.2016.03.001</a>

Result language

angličtina

Original language name

In vivo and in vitro effectiveness of Azadirachta indica-synthesized silver nanocrystals against Plasmodium berghei and Plasmodium falciparum, and their potential against malaria mosquitoes

Original language description

In this research, we synthesized silver nanoparticles (AgNP) using the Azadirachta indica seed kernel extract as reducing and stabilizing agent. AgNP were characterized by UV–vis spectrophotometry, SEM, EDX, XRD and FTIR spectroscopy. The A. indica seed kernel extract was toxic against Anopheles stephensi larvae and pupae, LC50 were 232.8 ppm (larva I), 260.6 ppm (II), 290.3 ppm (III), 323.4 ppm (IV), and 348.4 ppm (pupa). AgNP LC50 were 3.9 ppm (I), 4.9 ppm (II), 5.6 ppm (III), 6.5 ppm (IV), and 8.2 ppm (pupa). The antiplasmodial activity of A. indica seed kernel extract and AgNP was evaluated against CQ-resistant (CQ-r) and CQ-sensitive (CQ-s) strains of Plasmodium falciparum. IC50 of A. indica seed kernel extract were 63.18 µg/ml (CQ-s) and 69.24 µg/ml (CQ-r). A. indica seed kernel-synthesized AgNP achieved IC50, of 82.41 µg/ml (CQ-s) and 86.12 µg/ml (CQ-r). However, in vivo anti-plasmodial experiments conducted on Plasmodium berghei infecting albino mice showed moderate activity of the A. indica extract and AgNP. Overall, this study showed that the A. indica-mediated fabrication of AgNP is of interest for a wide array of purposes, ranging from IPM of mosquito vectors to the development of novel and cheap antimalarial drugs.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Research in Veterinary Science

ISSN

0034-5288

e-ISSN

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Volume of the periodical

106

Issue of the periodical within the volume

June

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

14-22

UT code for WoS article

000377923400003

EID of the result in the Scopus database

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