Comparison of Varroa destructor and Worker Honeybee Microbiota Within Hives Indicates Shared Bacteria

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003666" target="_blank" >RIV/00027006:_____/16:00003666 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00248-016-0776-y" target="_blank" >http://dx.doi.org/10.1007/s00248-016-0776-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00248-016-0776-y" target="_blank" >10.1007/s00248-016-0776-y</a>

Result language

angličtina

Original language name

Comparison of Varroa destructor and Worker Honeybee Microbiota Within Hives Indicates Shared Bacteria

Original language description

The ectoparasitic mite Varroa destructor is a major pest of the honeybee Apis mellifera. In a previous study, bacteria were found in the guts of mites collected from winter beehive debris and were identified using Sanger sequencing of their 16S rRNA genes. In this study, community comparison and diversity analyses were performed to examine the microbiota of honeybees and mites at the population level. The microbiota of the mites and honeybees in 26 colonies in seven apiaries in Czechia was studied. Between 10 and 50 Varroa females were collected from the bottom board, and 10 worker bees were removed from the peripheral comb of the same beehive. Both bees and mites were surface sterilized. Analysis of the 16S rRNA gene libraries revealed significant differences in the Varroa and honeybee microbiota. The Varroa microbiota was less diverse than was the honeybee microbiota, and the relative abundances of bacterial taxa in the mite and bee microbiota differed. The Varroa mites, but not the honeybees, were found to be inhabited by Diplorickettsia. The relative abundance of Arsenophonus, Morganella, Spiroplasma, Enterococcus, and Pseudomonas was higher in Varroa than in honeybees, and the Diplorickettsia symbiont detected in this study is specific to Varroa mites. The results demonstrated that there are shared bacteria between Varroa and honeybee populations but that these bacteria occur in different relative proportions in the honeybee and mite bacteriomes. These results support the suggestion of bacterial transfer via mites, although only some of the transferred bacteria may be harmful.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

<a href="/en/project/LH14060" target="_blank" >LH14060: Contribution of the microbial community of Varroa destructor to honeybee Colony Collapse Disorder</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microbial Ecology

ISSN

0095-3628

e-ISSN

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Volume of the periodical

72

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

448-459

UT code for WoS article

000379708500017

EID of the result in the Scopus database

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