Rapid diagnosis of two common stored-product predatory mite species based on species-specific PCR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003696" target="_blank" >RIV/00027006:_____/16:00003696 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jspr.2016.08.006" target="_blank" >http://dx.doi.org/10.1016/j.jspr.2016.08.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jspr.2016.08.006" target="_blank" >10.1016/j.jspr.2016.08.006</a>
Alternative languages
Result language
angličtina
Original language name
Rapid diagnosis of two common stored-product predatory mite species based on species-specific PCR
Original language description
Cheyletus malaccensis Oudemans and Cheyletus eruditus (Schrank) are two sibling species of the Cheyletus genus. Both species are beneficial predators of stored product pests. It is difficult to distinguish them based only on morphological characteristics. In this study, we describe species-specific PCR for molecular identification of C. malaccensis and C. eruditus. Species-specific primer pairs were designed on the basis of variations in the mtDNA COI barcode sequences. A 403 bp-specific fragment of C. malaccensis and a 421 bp absolute product of C. eruditus were generated by their respective primer pairs, while no such diagnostic bands were present in any of the nine pest mite species tested. The results of the sensitivity test demonstrated that the detection limit of DNA template concentration was 1 ng/µL for both C. malaccensis and C. eruditus. This study exhibited high specificity, reliability, rapid and low cost for mite specie diagnosis. Our data show that species-specific PCR is a powerful tool that can be used for identification of C. malaccensis and C. eruditus.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Stored Products Research
ISSN
0022-474X
e-ISSN
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Volume of the periodical
69
Issue of the periodical within the volume
October
Country of publishing house
GB - UNITED KINGDOM
Number of pages
4
Pages from-to
213-216
UT code for WoS article
000390514900032
EID of the result in the Scopus database
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