Detection of PCR Inhibition in Food and Feed with a Synthetic Plasmid
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F17%3A00003988" target="_blank" >RIV/00027006:_____/17:00003988 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.17221/374/2016-CJFS" target="_blank" >http://dx.doi.org/10.17221/374/2016-CJFS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/374/2016-CJFS" target="_blank" >10.17221/374/2016-CJFS</a>
Alternative languages
Result language
angličtina
Original language name
Detection of PCR Inhibition in Food and Feed with a Synthetic Plasmid
Original language description
The quantitative real-time polymerase chain reaction (qPCR) can be inhibited by various substances and the DNA content in the sample can be underestimated. It is necessary to identify PCR inhibition and choose an optimal DNA isolation protocol to correctly evaluate the sample. In a previous study, we have developed an assay using plasmid DNA carrying a non-homologous random sequence identifying possible inhibitors in qPCR in food/feed samples. Here, we present a successful use of the plasmid inhibition detection in and DNA isolation protocol optimisation for four food/feed samples in qPCR analysis of the sequence coding for chloroplast tRNA-Leu: two meat meal samples and two samples made of cranberries (jam and dried fruit). The plasmid assay allowed to effectively reveal PCR inhibition in all of the different sample matrices and to choose an optimal DNA isolation protocol.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
<a href="/en/project/QJ1530272" target="_blank" >QJ1530272: Complex strategies for effective detection of food fraud in the chain production-consumer</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Food Sciences
ISSN
1212-1800
e-ISSN
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Volume of the periodical
35
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
5
Pages from-to
160-164
UT code for WoS article
000400934000008
EID of the result in the Scopus database
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