New effective cryopreservation approach for grey poplar in vitro cultures

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F17%3A00004388" target="_blank" >RIV/00027006:_____/17:00004388 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
New effective cryopreservation approach for grey poplar in vitro cultures
Original language description
Populus x canescence (grey poplar) belongs among fast-growing woody plant species. For its unique phenotype traits observed in floodplain forest in the South Moravia region of the Czech Republic and a relatively rapid growth in in vitro as well, approximately 4-6 weeks of micropropagated plants were used to test the most effective culture conditions for a long-term conservation under ultra-low temperature. Firstly, excised apical and nodal segments were followed in their growth to determine suitable plant material for cryopreservation. Apical segments exhibited more steady developmental progress opposite to unbalanced growth of nodal segments therefore modified vitrification protocol (80% PVS3 cryoprotectant) was applied into the apical segments of 2 mm length.
Czech name
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Czech description
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Project
<a href="/en/project/QJ1630301" target="_blank" >QJ1630301: Development of the new biotechnological systems for maintanance and improvment of agriculture crop and forest tree biodiversitsy</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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