Alliinase and cysteine synthase transcription in developing garlic (Allium sativum L.) over time

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F18%3A00004821" target="_blank" >RIV/00027006:_____/18:00004821 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.foodchem.2017.12.090" target="_blank" >http://dx.doi.org/10.1016/j.foodchem.2017.12.090</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.foodchem.2017.12.090" target="_blank" >10.1016/j.foodchem.2017.12.090</a>

Result language
angličtina
Original language name
Alliinase and cysteine synthase transcription in developing garlic (Allium sativum L.) over time
Original language description
Garlic is a valuable source of health-promoting compounds, including secondary metabolites rich in sulfur such as cysteine sulfoxides (CSO). In this study, a new qRT-PCR assay is presented, analyzing the transcription of two genes encoding key enzymes of CSO biosynthetic pathway (cysteine synthase and alliinase) in developing garlic. In addition, a set of three reference genes (RGs) is presented which are suitable for normalization of the transcription of the described genes of interest. Using newly introduced qRT-PCR assays unique to cysteine synthase (chloroplast and cytosol specific), alliinase, and using RGs, we have shown that the corresponding genes have been actively transcribed throughout the whole life cycle with a peak in the germination phase. Since the content of sulfur-rich metabolites in garlic bulbs is based, among other factors, on the transcription of genes involved in the sulfur metabolism pathways in the leaves, this test can be used to examine the impact of agricultural practices or the environment on the content of sulfur metabolites in harvested garlic.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project
<a href="/en/project/QJ1210158" target="_blank" >QJ1210158: High quality and safe Allium vegetables focusing to garlic</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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