TaqMan Multiplex Real-Time qPCR assays for the detection and quantification of Barley yellow dwarf virus, Wheat dwarf virus and Wheat streak mosaic virus and the study of their interactions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F18%3A00005030" target="_blank" >RIV/00027006:_____/18:00005030 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1071/CP18189" target="_blank" >http://dx.doi.org/10.1071/CP18189</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1071/CP18189" target="_blank" >10.1071/CP18189</a>
Alternative languages
Result language
angličtina
Original language name
TaqMan Multiplex Real-Time qPCR assays for the detection and quantification of Barley yellow dwarf virus, Wheat dwarf virus and Wheat streak mosaic virus and the study of their interactions
Original language description
In the Czech Republic, Barley yellow dwarf virus (BYDV) and Wheat dwarf virus (WDV) are the prevailing viral species that can cause significant yield losses in cereal stands. Another virus, which has been on the increase in the Czech Republic and whose impact is expected to increase, is Wheat streak mosaic virus (WSMV). In this study, a TaqMan Multiplex Real-Time (qPCR) assay for simultaneous detection of BYDV (species PAV, PAS and MAV), WDV and WSMV was developed. Furthermore, additional assays for the viruses' quantification in wheat and barley were developed. The assay was optimised and used to identify possible synergistic or antagonistic relations between the viruses in wheat and barley which would be characterised by increases or decreases of the titre of the individual viruses. For this, a greenhouse experiment with artificial inoculations of mixed and single infections of BYDV-PAV, WDV and WSMV was carried out, and field samples from the wheat and barley stands with mixed and single infections of BYDV and WDV were collected. No trend of increasing or decreasing titre was recorded for any of the viruses apart from WSMV titre which was decreased approximately five times in mixed infections with BYDV or WDV compared with the single WSMV infection. Further analyses are needed to expand the idea of possible interactions between WSMV and WDV or BYDV.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/TF02000056" target="_blank" >TF02000056: Next generation sequencing (NGS) for the broad-spectrum diagnostics of plant viruses and for the study of virus-host interactions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Crop & Pasture Science
ISSN
1836-0947
e-ISSN
1836-5795
Volume of the periodical
69
Issue of the periodical within the volume
8
Country of publishing house
AU - AUSTRALIA
Number of pages
10
Pages from-to
755-764
UT code for WoS article
000441026700001
EID of the result in the Scopus database
2-s2.0-85052631914