Comparative de novo transcriptome analysis of barley varieties with different malting qualities

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10140668" target="_blank" >RIV/00027006:_____/20:10140668 - isvavai.cz</a>

Alternative codes found

RIV/60193697:_____/20:N0000019

Result on the web

<a href="https://link.springer.com/content/pdf/10.1007/s10142-020-00750-z.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007/s10142-020-00750-z.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10142-020-00750-z" target="_blank" >10.1007/s10142-020-00750-z</a>

Result language

angličtina

Original language name

Comparative de novo transcriptome analysis of barley varieties with different malting qualities

Original language description

Barley is one of the most important crops in the world. Barley is used as both food and feed and is important for malt production. Demands for malting quality differ among countries and customs. Malting quality is a complex characteristic involving barley genetics, the environmental conditions during barley growth, and the technological parameters of the malting process. In this study, the hypothesis was that there were no differences between two groups of barley varieties with different but defined malting qualities, which was tested using RNA sequencing during selected stages of malting. In total, 919 differentially transcribed genes between the two barley groups were identified and annotated. Differentially expressed genes (DEGs) were primarily assigned to gene ontology (GO) terms of oxidation-reduction process - oxidoreductase activity, response to stress, carbohydrate metabolic process, and proteolysis - hydrolase activity, and metal ion binding. Genes connected with the plasma membrane and its integral components also play important roles in malting quality. DEG profiles of selected genes in the three malting stages indicate a complex character of malting quality. Many single-nucleotide polymorphisms (SNPs) and insertions and deletions (indels) were identified. SNPs and indels with the best quality were used for primer design. After optimization and validation, five molecular markers were developed for use in barley breeding.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QK1910197" target="_blank" >QK1910197: The strategy for minimizing the impact of drought on sustainable production and barley malting quality</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FUNCTIONAL &amp; INTEGRATIVE GENOMICS

ISSN

1438-793X

e-ISSN

—

Volume of the periodical

20

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

12

Pages from-to

801-812

UT code for WoS article

000570837300001

EID of the result in the Scopus database

2-s2.0-85091136282