Methods of Thermal Analysis as a Tool to Develop Cryopreservation Protocols of Vegetatively Propagated Crops

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10144247" target="_blank" >RIV/00027006:_____/20:10144247 - isvavai.cz</a>
Result on the web
<a href="https://www.intechopen.com/books/cryopreservation-current-advances-and-evaluations/methods-of-thermal-analysis-as-a-tool-to-develop-cryopreservation-protocols-of-vegetatively-propagat" target="_blank" >https://www.intechopen.com/books/cryopreservation-current-advances-and-evaluations/methods-of-thermal-analysis-as-a-tool-to-develop-cryopreservation-protocols-of-vegetatively-propagat</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5772/intechopen.89723" target="_blank" >10.5772/intechopen.89723</a>

Result language
angličtina
Original language name
Methods of Thermal Analysis as a Tool to Develop Cryopreservation Protocols of Vegetatively Propagated Crops
Original language description
Cryopreservation is considered to be a reliable biotechnological tool for the long-term conservation of vegetatively propagated plant germplasm. The technique is based on freezing plant tissues at an ultra-low temperature. However, high water content in plant tissue can result in injury during the cooling and thawing processes. Water behaviour in the process of cryopreservation can be assessed by the use of thermal analysis method. This chapter demonstrates how the use of heat flux type differential scanning calorimetry (DSC) thermal analysis methods such as Standard DSC, Temperature Modulated DSC (TMDSC) and Quasi-Isothermal Temperature Modulated DSC (QITMDSC) can be used to assess the amount of freezable water and verify if the tissue being used has reached glass transition as well as analysing the thermal events during cooling and freezing to reduce crystallization and damage by frost. Here, you can find a guide on how these thermal analysis methods can be applied, through concrete examples of each method and their use in the development of a more reliable and precise cryopreservation protocol for vegetatively propagated plant species.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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