Cryoconservation of hop pollen (Humulus lupulus L.)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10149470" target="_blank" >RIV/00027006:_____/20:10149470 - isvavai.cz</a>

Alternative codes found

RIV/14864347:_____/19:N0000093 RIV/14864347:_____/19:N0000094 RIV/00027006:_____/19:00005667

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cryoconservation of hop pollen (Humulus lupulus L.)

Original language description

The hop plant, Humulus lupulus, L., is a dioecious perennial species, and only female clones are used for beer brewing. Cryopreservation of pollen is an additional way to conserve many species. In particular, it can be used as an alternative strategy to preserve the genetic variation and minimize problems of asynchronous flowering among accessions preserved in a germplasm bank (2017). Storage of pollen not only effectively overcomes the obstacles in crossing of parents flowering at different times and in different geographical locations, but also is an effective means to preserve the genetic diversity of plants. Multi-branched panicles of selected individuals of male hop clones (12/00, 12/06, 19/07) were collected in July 2019. Hop pollen grains were spontaneously released from panicles and they were collected into plastic test tubes in laboratory conditions. Thermal analysis of hop pollen was performed using differential scanning calorimeter (DSC) Q2000 (TA Instruments, USA) with refrigerated cooling system (RCS) in the temperature range from -90 to +20 deg.C. The cooling rate was 10 deg.C /min. Temperature-modulated DSC method was used during warming cycle with warming rate of 1 deg. C/min. Temperature modulation was performed at 1 deg. C amplitude of modulation and 60 second period. Aluminium, hermetically sealed pans were used, sample size ranged from 5 to 20 mg and the purge gas was nitrogen. No significant endothermic or exothermic events were detected in all genotypes tested. On the other hand, the glass transitions were detected in all samples during warming cycles and they ranged from -35 deg. C to -10 deg. C. The half-height of sample glass temperature was -18 deg. C, -20 deg. C and -28 deg. C in clones 19/07, 12/06 and 12/00, respectively. The determined thermal characteristics of all hop clones tested make their cryopreservation possible.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QK1910277" target="_blank" >QK1910277: The use of cryopreservation method to streamline breeding process of economically important agricultural crops and preservation of forest tree</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů