Real-time PCR for identification of five species of Cryptolestes based on COI barcode region

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10149493" target="_blank" >RIV/00027006:_____/20:10149493 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0022474X19305752/pdfft?md5=a1f0089f8339a651112e041568a09aab&pid=1-s2.0-S0022474X19305752-main.pdf" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0022474X19305752/pdfft?md5=a1f0089f8339a651112e041568a09aab&pid=1-s2.0-S0022474X19305752-main.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jspr.2020.101623" target="_blank" >10.1016/j.jspr.2020.101623</a>

Result language
angličtina
Original language name
Real-time PCR for identification of five species of Cryptolestes based on COI barcode region
Original language description
Cryptolestes: (Coleoptera: Laemophloeidae) are economically important pests, which damage stored products in granaries and mills. Because it is difficult to distinguish Cryptolestes species morphologically, we develop here a high sensitivity real-time PCR method for distinguishing five species of Cryptolestes(C. ferrugineus, C. pusillus, C. turcicus, C. pusilloides and C. capensis). The lower limit of DNA concentration required for species identification is 0.1 ng/ml for C. ferruginues and C. pusilloides, and 0.01 ng/ml for C. pusillus, C. turcicus, and C. capensis. This method successfully determined species assignments for previously unidentified Cryptolestes spp. Collected from the field and will facilitate Cryptolestes identification in the field of plant quarantine and stored product protection.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project
<a href="/en/project/LTACH19029" target="_blank" >LTACH19029: The invasive mechanisms of economically important stored insect pests endangering Chinese and European international trades and phytosanitary technical systems for their managment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)