A scientific note on the comparison of PCR based quantification methods of Melissococcus plutonius in honey bees

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10149609" target="_blank" >RIV/00027006:_____/20:10149609 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10442458 RIV/60460709:41210/20:88583
Result on the web
<a href="https://www.tandfonline.com/toc/tjar20/60/2" target="_blank" >https://www.tandfonline.com/toc/tjar20/60/2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/00218839.2020.1844466" target="_blank" >10.1080/00218839.2020.1844466</a>

Result language
angličtina
Original language name
A scientific note on the comparison of PCR based quantification methods of Melissococcus plutonius in honey bees
Original language description
Melissococcus plutonius is the causative agent of European foulbrood of honey bee larvae (Apis mellifera). The early diagnosis of disease is necessary for its elimination. Previously, we found false-positive results for PCR detection of M. plutonius in honey bee workers by qPCR. Here we report the comparison of PCR methods for quantification of M. plutonius in the bacteria spiked samples of honey bee workers and detection limits to eliminate false-positive results. We compare available primers of qPCR and PCR detection. The obtained detection limit was 10(2) copies of M. plutonius. The results of this comparison show conventional PCR detection using EFB_primers as a suitable method to confirm visual observation of European foulbrood using samples of honey bee workers. Based on our estimation, the numbers of M. plutonius should be quantified by copies of the Na thorn /H thorn 50 antiporter marker (MP_primers) in honey bee worker samples
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project
<a href="/en/project/TH02030317" target="_blank" >TH02030317: Developing of detection set for European foulbrood of honey bees</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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