Microbiome variation during culture growth of the European house dust mite, Dermatophagoides pteronyssinus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F21%3A10149595" target="_blank" >RIV/00027006:_____/21:10149595 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/21:88582 RIV/00216224:14310/21:00122404

Result on the web

<a href="https://academic.oup.com/femsec/issue/97/4" target="_blank" >https://academic.oup.com/femsec/issue/97/4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsec/fiab039" target="_blank" >10.1093/femsec/fiab039</a>

Result language

angličtina

Original language name

Microbiome variation during culture growth of the European house dust mite, Dermatophagoides pteronyssinus

Original language description

In culture, the house dust mite, Dermatophagoides pteronyssinus, shows different growth patterns, but the composition and changes in the associated microbial community during mite culture growth are poorly known. In this study, we analyzed temporal changes in microbial communities including &apos;internal&apos; communities (inside mites, ingested) and &apos;environmental&apos; communities (from culture environment). Microbial community structure was correlated with guanine content (a nitrogenous waste product of mites) and mite population density. Both internal and environmental microbial communities were remarkably consistent between biological replicates from the same culture age group and were composed of relatively few dominant taxa-11 bacterial and 3 fungal operational taxonomic units (OTUs). Significant changes over time in microbial community structure in the bulk culture environment and in internal mite samples were observed. The yeast, Saccharomyces cerevisiae, a main component of the mite diet, gradually disappeared during mite culture growth and was replaced by fungi from the genera Aspergillus and Candida in both &apos;internal&apos; and &apos;environmental&apos; samples. In environmental samples, bacteria from the genus Lactobacillus and S. cerevisiae were negatively correlated, and Aspergillus and Candida positively correlated, with guanine content. The relative abundance of bacteria from the genus Kocuria increased with mite density but declined with increasing guanine content. The relative abundance of bacteria from the genus Virgibacillus was negatively correlated with mite density in &apos;internal&apos; samples. Gram-positive bacteria dominated bacterial microbiomes at all time points in our experiments, indicating a more limited possibility for vaccine contamination by bacterial endotoxins (heat-stable lipopolysaccharides produced mostly by Gram-negative bacteria) in our experimental cultures.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/GA17-12068S" target="_blank" >GA17-12068S: Are the associated bacteria and waste metabolite responsible for the decline of populations house dust mites?</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEMS MICROBIOLOGY ECOLOGY

ISSN

0168-6496

e-ISSN

—

Volume of the periodical

97

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

16

Pages from-to

fiab039

UT code for WoS article

000644508800016

EID of the result in the Scopus database

2-s2.0-85103683715