Morphological, Genetic and Biochemical Evaluation of Dasypyrum villosum (L.) P. Candargy in the Gene Bank Collection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F21%3A10174294" target="_blank" >RIV/00027006:_____/21:10174294 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2073-4395/11/7/1316/pdf" target="_blank" >https://www.mdpi.com/2073-4395/11/7/1316/pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/agronomy11071316" target="_blank" >10.3390/agronomy11071316</a>
Alternative languages
Result language
angličtina
Original language name
Morphological, Genetic and Biochemical Evaluation of Dasypyrum villosum (L.) P. Candargy in the Gene Bank Collection
Original language description
The Dasypyrum villosum gene bank collection, comprising 32 accessions, was characterized morphologically and genetically for resistance to leaf diseases and for quality parameters of seeds with specific accent to protein polymorphism and protein and starch composition. The collected material represented nearly the whole distribution area in the Mediterranean. For SSR analysis, a set of 40 SSR markers for wheat was selected. A matrix of distances between genotypes was calculated using Simple Matching dissimilarity coefficient in the DARwin software. The collection was scored for resistance to powdery mildew, brown, stripe and stem rusts. A modified SDS-PAGE method with clear interpretation of high and low molecular glutenin subunits (HMW, LMW) was used for characterization of accessions. Morphological phenotyping revealed considerable diversity allowing the distinguishing of clusters tracing the geographical origin of accessions. Genetic diversity showed three groups but without significant bootstrap support. All tested accessions were resistant to the applied races of powdery mildew and leaf rust. Three accessions were moderately susceptible to currently available races of yellow rust. Biochemical analyses of seeds in selected populations showed a high content of crude proteins with a significant proportion of prolamins and Sigma glutelins. The SDS-PAGE of HMW and LMW glutelins confirmed both the high population polymorphism and the intra-population differences. Apart from the recent research in CWR breeding, Dasypyrum villosum is still an underrepresented species in germplasm collections and an underutilized species in breeding.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Agronomy-Basel
ISSN
2073-4395
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
7
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
1316
UT code for WoS article
000675940700001
EID of the result in the Scopus database
2-s2.0-85109358219