Delayed Leaf Senescence by Upregulation of Cytokinin Biosynthesis Specifically in Tomato Roots
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F22%3A10175136" target="_blank" >RIV/00027006:_____/22:10175136 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15640/22:73618606
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fpls.2022.922106/pdf" target="_blank" >https://www.frontiersin.org/articles/10.3389/fpls.2022.922106/pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fpls.2022.922106" target="_blank" >10.3389/fpls.2022.922106</a>
Alternative languages
Result language
angličtina
Original language name
Delayed Leaf Senescence by Upregulation of Cytokinin Biosynthesis Specifically in Tomato Roots
Original language description
Cytokinins (CKs) regulate numerous plant developmental processes, including photosynthesis and leaf senescence. Isopentenyltransferase (IPT) is a rate-limiting enzyme in the CK-biosynthesis pathway. We overexpressed ipt under tissue-specific promoters to study the long-range effect of CK on the functioning of tomato source leaves. Photosynthetic activity over time provided the measure for leaf aging. Significantly delayed leaf senescence was observed in plants expressing ipt under a root-specific promoter, but not in those expressing the gene under a source leaf-specific promoter. The root-derived influence on leaf aging was further confirmed by grafting experiments. CK concentration in source leaves of both transgenic lines increased significantly, with different proportions of its various derivatives. On the other hand, root CK concentration was only slightly elevated. Nevertheless, the significant change in the proportion of CK derivatives in the root indicated that CK biosynthesis and metabolism were altered. Partial leaf defoliation upregulates photosynthetic rate in the remaining leaf; however, overexpression of ipt in either tissues eliminated this response. Interestingly, stem girdling also eliminated the photosynthetic response. Taken together, our findings suggest that leaf senescence is regulated by a CK-mediated root-shoot communication network. We propose that CK-mediated signal is translocated to the leaf via the xylem where it alters CK biosynthesis, resulting in delayed senescence.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Plant Science
ISSN
1664-462X
e-ISSN
—
Volume of the periodical
13
Issue of the periodical within the volume
JUL 6 2022
Country of publishing house
CH - SWITZERLAND
Number of pages
10
Pages from-to
922106
UT code for WoS article
000829446000001
EID of the result in the Scopus database
2-s2.0-85134543354