Proteomic insight into the interaction of Paenibacillus larvae with honey bee larvae before capping collected from an American foulbrood outbreak: pathogen proteins within the host, lysis signatures and interaction markers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176080" target="_blank" >RIV/00027006:_____/23:10176080 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/23:10472668

Result on the web

<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/toc/16159861/2023/23/1" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/toc/16159861/2023/23/1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/pmic.202200146" target="_blank" >10.1002/pmic.202200146</a>

Result language

angličtina

Original language name

Proteomic insight into the interaction of Paenibacillus larvae with honey bee larvae before capping collected from an American foulbrood outbreak: pathogen proteins within the host, lysis signatures and interaction markers

Original language description

American foulbrood (AFB) is a devastating disease of honey bees. There remains a gap in the understanding of the interactions between the causative agent and host, so we used shotgun proteomics to gain new insights. Nano-LC-MS/MS analysis preceded visual description and Paenibacillus larvae identification in the same individual sample. A further critical part of our methodology was that larvae before capping were used as the model stage. The identification of the virulence factors SplA, PlCBP49, enolase, and DnaK in all P. larvae-positive samples was consistent with previous studies. Furthermore, the results were consistent with the array of virulence factors identified in an in vitro study of P. larvae exoprotein fractions. Although an S-layer protein and a putative bacteriocin were highlighted as important, the microbial collagenase ColA and InhA were not found in our samples. The most important virulence factor identified was isoform of neutral metalloproteinase (UniProt: V9WB82), a major protein marker responsible for the shift in the PCA biplot. This protein is associated with larval decay and together with other virulence factors (bacteriocin) can play a key role in protection against secondary invaders. Overall, this study provides new knowledge on host-pathogen interactions and a new methodical approach to study the disease.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QK1710228" target="_blank" >QK1710228: New reliable methods for routine differentiation of strains and prediction of development and spread of the causative agent of American foulbrood (Paenibacillus larvae)</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PROTEOMICS

ISSN

1615-9853

e-ISSN

—

Volume of the periodical

23

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

e2200146

UT code for WoS article

000878315800001

EID of the result in the Scopus database

2-s2.0-85141362057