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Methodology for co-cryostorage of virus isolates in their host plants and relevant data

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F23%3A10176441" target="_blank" >RIV/00027006:_____/23:10176441 - isvavai.cz</a>

  • Alternative codes found

    RIV/60077344:_____/23:00584637

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Methodology for co-cryostorage of virus isolates in their host plants and relevant data

  • Original language description

    Currently, the nursery production of plant material emphasizes the production of healthy, virus-free material. For this reason, it is necessary to have reference plant materials in which the virus is present. Some types of viruses are difficult to maintain outside the plant host. Because of the potential transmission to outdoor plantings, it is necessary to keep infected plants in special cultivation areas, and net-houses, which brings considerable financial and labour costs. Therefore, a methodology was developed in which the virus status in plant material is analysed, the infected material can be stored using cryopreservation in liquid nitrogen and then regenerated in vitro, and relevant data in GeneEver cryobox can be stored.

  • Czech name

  • Czech description

Classification

  • Type

    N<sub>metS</sub> - Methodology approved by the given body of the state whose it is competency

  • CEP classification

  • OECD FORD branch

    40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Result continuities

  • Project

    <a href="/en/project/TO01000295" target="_blank" >TO01000295: Healthy berries in a changing climate: development of new biotechnological procedures for virus diagnostics, vector studies, elimination and safe preservation of strawberry and raspberry</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Internal product ID

    7/23

  • Regulation ID

    MZE-73887/2023-13113

  • Technical parameters

    Osvědčení o uznání metodiky č.j. MZE-73887/2023-13113 vydalo Ministerstvo zemědělství, Odbor environmentálního a ekologického zemědělství dne 22.12.2023. Smlouva o uplatnění metodiky č. 18/2023 byla uzavřena dne20.12.2023 mezi Výzkumným ústavem rostlinné výroby, v.v.i., Drnovská 507/73, 161 06 Praha 6, IČ: 00027006, DIČ: CZ00027006 (poskytovatel) a Ministerstvo zemědělství, Odbor environmentální a ekologického zemědělství, Těšnov 65/17, 110 00 Praha 1, IČ: 00020478, DIČ: CZ 00020478 zastoupené Ing. Vlastimilem Zedkem, ředitelem odboru (uživatel). Statutární zástupce za VÚRV, v.v.i.: RNDr. Mikuláš Madaras, ředitel. Kontaktní osoba za VÚRV, v.v.i.: RNDr. Alios Bilavčík, Ph.D., tel.: 233022433, e-mail: bilavcik@vurv.cz ISBN (PDF): 978-80-7427-417-6

  • Economical parameters

    Cryopreservation is economically beneficial, saving storage space and preserving material for biotech applications. Co-storing virus isolates with data reduces the risk of loss, a key advantage for users.

  • Certification body designation

    Ministerstvo zemědělství, Odbor environmentálního a ekologického zemědělství, Těšnov 65/17, 110 00 Praha 1

  • Date of certification

  • Method of use

    C - Výsledek je využíván bez omezení okruhu uživatelů