Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F24%3A10177102" target="_blank" >RIV/00027006:_____/24:10177102 - isvavai.cz</a>
Result on the web
<a href="https://cjgpb.agriculturejournals.cz/pdfs/cjg/2024/02/03.pdf" target="_blank" >https://cjgpb.agriculturejournals.cz/pdfs/cjg/2024/02/03.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/84/2023-CJGPB" target="_blank" >10.17221/84/2023-CJGPB</a>
Alternative languages
Result language
angličtina
Original language name
Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)
Original language description
Several antimitotic agents were tested in three embryogenic doubled haploid (DH) lines of swede (Brassica napus ssp. napobrassica). No effect on embryogenesis was observed at the given concentrations of colchicine (5 mu mol/L or 50 mg/L) and treatment time of 24 hours. Flow cytometric analysis of microspore embryos revealed a significant increase in the percentage of DH compared to the control for all substances: ethalfluralin, 62.2%; trifluralin, 58.3%; colchicine, 56.1%; amiprophos-methyl, 54.4%; pronamide, 35.0%; control, 13.3%. The occurrence of pure tetraploids was low (0-5%), mixoploids especially n+2n were high in all treatments, including the control (29.4-71.1%). Their presence can be explained as a consequence of spontaneous diploidisation and/or endoreduplication during embryo genesis or embryo germination.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/QK1910070" target="_blank" >QK1910070: Utilization of biotechnological methods and non-traditional genetic resources for the characterization and construction of uniform lines of cruciferous vegetables with specific parameters of quality, yield and resistance to significant diseases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Genetics and Plant Breeding
ISSN
1212-1975
e-ISSN
1805-9325
Volume of the periodical
60
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
79-85
UT code for WoS article
001136370800001
EID of the result in the Scopus database
2-s2.0-85189289710