A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomato
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F24%3A10177405" target="_blank" >RIV/00027006:_____/24:10177405 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15640/24:73626026
Result on the web
<a href="https://academic.oup.com/jxb/article-pdf/75/16/5021/58890850/erae210.pdf" target="_blank" >https://academic.oup.com/jxb/article-pdf/75/16/5021/58890850/erae210.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/erae210" target="_blank" >10.1093/jxb/erae210</a>
Alternative languages
Result language
angličtina
Original language name
A dual regulatory role for the arbuscular mycorrhizal master regulator RAM1 in tomato
Original language description
The REQUIRED FOR ARBUSCULAR MYCORRHIZATION1 (RAM1) transcription factor from the GRAS family is well known for its role as a master regulator of the arbuscular mycorrhizal (AM) symbiosis in dicotyledonous and monocotyledonous species, being essential in transcriptional reprogramming for the development and functionality of the arbuscules. In tomato, SlGRAS27 is the putative orthologue of RAM1 (here named SlRAM1), but has not yet been characterized. A reduced colonization of the root and impaired arbuscule formation were observed in SlRAM1-silenced plants, confirming the functional conservation of the RAM1 orthologue in tomato. However, unexpectedly, SlRAM1-overexpressing (UBIL:SlRAM1) plants also showed decreased mycorrhizal colonization. Analysis of non-mycorrhizal UBIL:SlRAM1 roots revealed an overall regulation of AM-related genes and a reduction of strigolactone biosynthesis. Moreover, external application of the strigolactone analogue GR244DO almost completely reversed the negative effects of SlRAM1 overexpression on the frequency of mycorrhization. However, it only partially recovered the pattern of arbuscule distribution observed in control plants. Our results strongly suggest that SlRAM1 has a dual regulatory role during mycorrhization and, in addition to its recognized action as a positive regulator of arbuscule development, it is also involved in different mechanisms for the negative regulation of mycorrhization, including the repression of strigolactone biosynthesis. This is the first time a dual role (positive and negative) for RAM1 during mycorrhization has been demonstrated, prompting further exploration into the role of RAM1 across different plant species.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF EXPERIMENTAL BOTANY
ISSN
0022-0957
e-ISSN
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Volume of the periodical
75
Issue of the periodical within the volume
16
Country of publishing house
GB - UNITED KINGDOM
Number of pages
16
Pages from-to
5021-5036
UT code for WoS article
001263022400001
EID of the result in the Scopus database
2-s2.0-85202538232