High genetic variability of Puccinia triticina at a specific location in Slovakia: a case study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10179237" target="_blank" >RIV/00027006:_____/25:10179237 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/journal/42976/volumes-and-issues/53-3" target="_blank" >https://link.springer.com/journal/42976/volumes-and-issues/53-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s42976-024-00624-1" target="_blank" >10.1007/s42976-024-00624-1</a>

Result language

angličtina

Original language name

High genetic variability of Puccinia triticina at a specific location in Slovakia: a case study

Original language description

Leaf rust, caused by Puccinia triticina, is the most common and worldwide-distributed disease of wheat cultivated everywhere. Worldwide, the variability of P. triticina isolates is large, and molecular detection of their polymorphism using SSR targeting Slovak isolates has not been carried out so far. For this reason, this study dealt with polymorphism detection using SSR (simple sequence repeat) combined with virulence analysis and statistical processing of thirty leaf rust isolates collected from one location in Slovakia in 2020. This location is specific because several hundred cereal genetic resources have been yearly propagated there since 1996. Twenty-two virulence phenotypes and thirty SSR genotypes of P. triticina were detected. Resistance genes strongly influencing the virulence variability of the isolates in the given location were mainly Lr1, Lr15, Lr24, Lr26, Lr2c, and Lr2b. All isolates were virulent against Lr3a and Lr13, and avirulent to Lr9 and Lr19, so these genes did not influence pathogen virulence variability. Using 15 SSR primers, 60 different alleles were detected, with an average of 4 alleles per locus. The SSR markers PtSSR68, PtSSR154, and RB11 had the greatest ability to detect polymorphism in Slovak leaf rust isolates in this location. Based on structure analysis, a set of 30 P. triticina isolates was divided into two groups. These groups differed in the degree of genetic variability of individual isolates, and their difference was statistically significant. A high number of heterozygous alleles, significant deviation from Hardy-Weinberg equilibrium, and no Mantel correlation between genetic and virulence variability revealed the presence of clonal reproduction of the P. triticina. These results regarding the high genetic variability detected in Slovak isolates collected from one location may be due to the sampling site, where hundreds of genetic resources of cereals, primary, and potential P. triticina hosts have been propagated for decades.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QK22010298" target="_blank" >QK22010298: Application of a new Genomic selection approach in wheat breeding</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

CEREAL RESEARCH COMMUNICATIONS

ISSN

0133-3720

e-ISSN

—

Volume of the periodical

53

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

1505-1517

UT code for WoS article

001390911500001

EID of the result in the Scopus database

2-s2.0-85214417238