Development of a Reverse-Transcription Loop-Mediated Isothermal Amplification Assay for Detecting Brassica Yellows Virus in China
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10179518" target="_blank" >RIV/00027006:_____/25:10179518 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2073-4395/15/12/2727/pdf" target="_blank" >https://www.mdpi.com/2073-4395/15/12/2727/pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/agronomy15122727" target="_blank" >10.3390/agronomy15122727</a>
Alternative languages
Result language
angličtina
Original language name
Development of a Reverse-Transcription Loop-Mediated Isothermal Amplification Assay for Detecting Brassica Yellows Virus in China
Original language description
Brassica yellows virus (BrYV) mainly infects cruciferous crops and has been widely prevalent across China. To develop a rapid and highly sensitive method for detecting BrYV in oilseed rape, a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was established. Four specific primers were designed to target the conserved gene of BrYV, with total RNA extracted from BrYV-infected oilseed rape leaves used as the template for the RT-LAMP assay. The optimal reaction conditions were determined, including a primer concentration ratio of 1:8, MgSO4 concentration of 4 mM, reaction temperature of 64 ◦C, and a suitable reaction time of 60 min. Sensitivity analysis demonstrated that the RT-LAMP assay could detect total RNA at a concentration of 0.091 × 10−3 μg/μL, which was 100-fold more sensitive than conventional RT-PCR for BrYV detection. In addition to visualizing results by electrophoresis, the RT-LAMP assay could also be easily visualized using calcein-MnCl2. These results indicate the potential of the established RT-LAMP assay for rapid BrYV detection in oilseed rape plants, which can provide better technical support for field diagnosis, disease forecasting, and the implementation of effective control strategies against the virus.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Agronomy-Basel
ISSN
2073-4395
e-ISSN
2073-4395
Volume of the periodical
15
Issue of the periodical within the volume
12
Country of publishing house
CH - SWITZERLAND
Number of pages
8
Pages from-to
2727
UT code for WoS article
001646088500001
EID of the result in the Scopus database
2-s2.0-105027531155