Molecular mechanisms of insulin-like growth factor 1 promoted synthesis and retention of hyaluronic acid in porcine oocyte-cumulus complexes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F07%3A%230000423" target="_blank" >RIV/00027014:_____/07:#0000423 - isvavai.cz</a>
Alternative codes found
RIV/67985904:_____/07:00088689
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Molecular mechanisms of insulin-like growth factor 1 promoted synthesis and retention of hyaluronic acid in porcine oocyte-cumulus complexes
Original language description
The purpose of this study was to elucidate signaling pathways by which insulin like-growth factor 1 (IGF1) promotes FSH-stimulated synthesis and retention of hyaluronic acid (HA) in pig oocyt cumulus complexes (OCCs) cultured in serum-free medium. IGF1 had no effect on FSH-stimulated production of cAMP and activation of protein kinase A in the OCCs. The exposure of OCCs to both FSH and IGF1 resulted in a significant (P<0.05) increase in AKT and MAPK3/1 phosphorylation. An inhibitor of phosphoinositide-3-kinase (PIK3) LY 294002 significantly (P<0.05) reduced the IGF1 enhanced phosphorylation of AKT and inhibitors of AKT (SH6) and MAPK3/1 (U0126) significantly (P<0.05) decreased synthesis and retention of HA stimulated by parallel exposure of OCCs to both FSH and IGF1. The IGF1-promoted synthesis of HA was not accompanied by an increase in the relative abundance of hyaluronan synthase 2 (HAS2 mRNA in the cumulus cells. We conclude that IGF1 promotes the FSH-stimulated synthesi and rete
Czech name
Molekulární mechanismy IGF-1 stimulované syntézy kyseliny hyaluronové v prasečích kumulus-oocyt komplexech.
Czech description
Cílem práce bylo objasnění signálních mechanismů kterými IGF-1 podporuje FSH-stimulovanou syntézu a retenci kyseliny hyaluronové (HA) v oocyte-cumulus komplexech (OCC) u prasete. IGF-1 neovlivnil FSH stimulovanou produkci cAMP a aktivaci protein kinasy A(AKT). Vzájemné působení FSH a IGF-1 mělo za následek signifikantní vzestup AKT a fosforylaci MAPK3/1. tyto efekty byl významně inhibovány LY294002 a U0126. IGF-1 podporovaná syntéza HA nebyla doprovázena vzestupem relativního přebytku hyaluronan syntázy (HAS2 mRNA) v kumulárních buňkách. Závěrem je konstatováno že IGF-1 podporuje FSH-stimulovanou syntézu a retenci HA v prasečích OCC pomocí PIK3/AKT a MAPK3/1 dependentních mechanismů.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA523%2F04%2F0574" target="_blank" >GA523/04/0574: Role of luteinizing hormone receptors in maturationof pig oocyte-cumulus cell complexes in vivo and in vitro</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biology of Reproduction
ISSN
0006-3363
e-ISSN
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Volume of the periodical
76
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
1016-1024
UT code for WoS article
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EID of the result in the Scopus database
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