Assessment of the plasma membrane resistance of boar spermatozoa with using short hypoosmotic swelling test

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F09%3A%230001117" target="_blank" >RIV/00027014:_____/09:#0001117 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Assessment of the plasma membrane resistance of boar spermatozoa with using short hypoosmotic swelling test

Original language description

The objective of this study was to assess the sperm membrane resistance in diluted boar semen with using short hypoosmotic swelling test (sHOS-test). sHOS-test evaluates the functional integrity of the spermatozoa plasma membrane, especially of the tail.sHOS-test involves incubation of spermatozoa in hypoosmotic solution 75 mOsm/l for 5 min. Eosin-nigrosine staining test was used with sHOS-test. Ejaculates from 21 fertile hybrid AI boars were collected by the gloved hand method. In the fresh boar semenwere evaluated these parameters: semen volume, sperm motility, sperm concentration, morphologically abnormal spermatozoa, percentage of live sperm and sHOS-test. The boar semen was diluted in a semendilution rate of 1 + 4 in Androhep, Androstar, Androstar plus, MIII, LD and was stored at a temperature 17°C up to 96h. Samples of semen-dilution were kept at 38°C in water bath and resistance of spermatozoa was evaluated with sHOS-test before and after 1h incubation in water bath each day.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GI - Farm animal breeding and farm animal pedigree breeding

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Research in Pig Breeding

ISSN

1802-7547

e-ISSN

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Volume of the periodical

3

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

3

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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