Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F12%3A%230001634" target="_blank" >RIV/00027014:_____/12:#0001634 - isvavai.cz</a>
Result on the web
<a href="http://www.vuzv.cz/sites/File/_privat/12075.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/12075.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.livsci.2011.12.018" target="_blank" >10.1016/j.livsci.2011.12.018</a>
Alternative languages
Result language
angličtina
Original language name
Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis
Original language description
Genetic polymorphisms of bovine ?-casein (CSN3) and ?-lactoglobulin (LGB) have been shown to affect milk compositional traits and technological properties. Monitoring individual CSN3 and LGB genetic variants in the population is important in the subsequent process of breeding cattle and improving milk protein quality. The objective of this study was to develop and validate a method for genotyping the main mutations of the bovine CSN3 and LGB genes using real time PCR (RT-PCR) followed by a high-resolution melting method (HRM) assay. The HRM-based assay allowed the sensitive and fast identification of the CSN3 AA, AB, BB, AE and BE genotypes and LGB AA, AB and BB genotypes simultaneously. This method was validated by analyzing 100 Fleckvieh cattle DNA samples, which were also genotyped by conventional methods at the molecular level using PCR?RFLP and DNA sequencing techniques. The genotypes obtained using the HRM approach perfectly matched the genotypes obtained by conventional analyses
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Livestock Science
ISSN
1871-1413
e-ISSN
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Volume of the periodical
145
Issue of the periodical within the volume
1-3
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
5
Pages from-to
275-279
UT code for WoS article
000303270400037
EID of the result in the Scopus database
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