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Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F12%3A%230001634" target="_blank" >RIV/00027014:_____/12:#0001634 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.vuzv.cz/sites/File/_privat/12075.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/12075.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.livsci.2011.12.018" target="_blank" >10.1016/j.livsci.2011.12.018</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis

  • Original language description

    Genetic polymorphisms of bovine ?-casein (CSN3) and ?-lactoglobulin (LGB) have been shown to affect milk compositional traits and technological properties. Monitoring individual CSN3 and LGB genetic variants in the population is important in the subsequent process of breeding cattle and improving milk protein quality. The objective of this study was to develop and validate a method for genotyping the main mutations of the bovine CSN3 and LGB genes using real time PCR (RT-PCR) followed by a high-resolution melting method (HRM) assay. The HRM-based assay allowed the sensitive and fast identification of the CSN3 AA, AB, BB, AE and BE genotypes and LGB AA, AB and BB genotypes simultaneously. This method was validated by analyzing 100 Fleckvieh cattle DNA samples, which were also genotyped by conventional methods at the molecular level using PCR?RFLP and DNA sequencing techniques. The genotypes obtained using the HRM approach perfectly matched the genotypes obtained by conventional analyses

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Livestock Science

  • ISSN

    1871-1413

  • e-ISSN

  • Volume of the periodical

    145

  • Issue of the periodical within the volume

    1-3

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    5

  • Pages from-to

    275-279

  • UT code for WoS article

    000303270400037

  • EID of the result in the Scopus database