Factors affecting the number of STRO-1(+) stem cells derived from regenerating antler and pedicle cells of red and fallow deer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F12%3A%230001645" target="_blank" >RIV/00027014:_____/12:#0001645 - isvavai.cz</a>

Result on the web

<a href="http://www.vuzv.cz/sites/File/_privat/12087.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/12087.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1071/AN12012" target="_blank" >10.1071/AN12012</a>

Result language

angličtina

Original language name

Factors affecting the number of STRO-1(+) stem cells derived from regenerating antler and pedicle cells of red and fallow deer

Original language description

Mesenchymal stem cells positive to surface antigen STRO-1 were isolated from regenerating antlers of red deer (Cervus elaphus) and fallow deer (Dama dama) using a magnetic-activated cell sorting (MACS) method. In this study we analysed factors potentially affecting the number of STRO-1(+) cells in the cell cultures. With regard to the STRO-1 antigen, we evaluated data from 188 MACS separation procedures of cell cultures cultivated in Dulbecco's Modified Eagle Medium and 10% fetal calf serum of four fallow deer males (130 procedures) and four red deer males (58 procedures). The analysed factors were the sampling site of the antler or the pedicle, cell passage and type of the cell culture (mixed or STRO-1 negative cell cultures). The percentage of obtained STRO-1(+) cells varied greatly from 0.4 to 38.9% for fallow deer and from 1.8 to 16.5% for red deer. We have not found any significant influence of the sampling site. The passage and the type of culture were significant factors for bot

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA523%2F08%2F0808" target="_blank" >GA523/08/0808: Hormonal base of the relationship between social activity and antler growth III</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animal Production Science 

ISSN

1836-0939

e-ISSN

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Volume of the periodical

52

Issue of the periodical within the volume

8

Country of publishing house

AU - AUSTRALIA

Number of pages

4

Pages from-to

746-749

UT code for WoS article

000306282400012

EID of the result in the Scopus database

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