Analyzing the exome heterogeneity of cattle immunity genes with the method of flow-cell sequencing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F20%3AN0000183" target="_blank" >RIV/00027014:_____/20:N0000183 - isvavai.cz</a>
Result on the web
<a href="https://www.bio-conferences.org/articles/bioconf/full_html/2020/01/bioconf_fies2020_00247/bioconf_fies2020_00247.html" target="_blank" >https://www.bio-conferences.org/articles/bioconf/full_html/2020/01/bioconf_fies2020_00247/bioconf_fies2020_00247.html</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1051/bioconf/20201700247" target="_blank" >10.1051/bioconf/20201700247</a>
Alternative languages
Result language
angličtina
Original language name
Analyzing the exome heterogeneity of cattle immunity genes with the method of flow-cell sequencing
Original language description
Toll-like receptors belong to the pattern-recognition receptors (PRRs), which have evolved to recognize conserved features of bacterial and viral molecules. We used the approach developed earlier to screen for the polymorphism in TLR genes in a representative set of historical and modern cattle breeds from Russia. The method pipeline included the steps of obtaining the overlapping amplification products from the coding regions of all ten bovine TLR genes, their subsequent purification and normalization. While the anti-bacterial group included TLR1, -2, -4, -5 and -6, the anti-viral group compressed TLR3, -7, -8, -9 and -10 (in spite of its unclear specificity). Animals from the about seven breeds, both bulls and cows, was used for analysis. The samples from the pooled genomic DNA were sequenced on the PacBio platform. After identification of variations, Bayesian analysis was carried out, followed by filtration on quality of sequencing. The 5-36 structural variants of TLRs were annotated according to their biological significance. Both new and already identified sites of variability, already annotated and documented in dbSNP, have been found. The data are needed for further breeding of local breeds in Russia with respect to their natural resistance to various diseases.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
40203 - Husbandry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
BIO Web of Conferences 17, 00247 (2020)
ISBN
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ISSN
2117-4458
e-ISSN
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Number of pages
5
Pages from-to
Article Number 00247
Publisher name
International Scientific-Practical Conference “Agriculture and Food Security: Technology, Innovation, Markets, Human Resources” (FIES 2019)
Place of publication
Bio Web Conf. Volume 17, 2020
Event location
Kazaň, Rusko
Event date
Nov 13, 2019
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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