Milos Beran. Invited lecture: Continuous enzyme reactors equipped with submicron fiber membranes for enzyme immobilisation, BIT’s 9th Annual World Congress of Nano Science &Technology (Nano S&T-2019). October 20-22, 2019, Suzhou, Čína.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F19%3AN0000003" target="_blank" >RIV/00027022:_____/19:N0000003 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Milos Beran. Invited lecture: Continuous enzyme reactors equipped with submicron fiber membranes for enzyme immobilisation, BIT’s 9th Annual World Congress of Nano Science &Technology (Nano S&T-2019). October 20-22, 2019, Suzhou, Čína.

Original language description

While most nanofibers are produced using electrospinning, this technique suffers from several drawbacks, such as the requirement for specialized equipment, high electrical potential, and electrically conductive targets. Consequently, recent years have seen the increasing emergence of novel strategies in generating nanofibers in a larger scale and higher throughput manner. The nozzleless centrifugal spinning is simple, cheap and highly productive technology for nanofiber production. In principle, the drawing of solution filament into nanofibers using centrifugal spinning is achieved through the controlled manipulation of centrifugal force, viscoelasticity, and mass transfer characteristics of the spinning solutions. We have used a pilot plant demonstrator Nanocent of the nozzleless centrifugal spinning to produce polyhydroxybutyrate (P3HB) submicron fiber membranes. Polyhydroxyalkanoates (PHAs) belong among the promising materials for enzyme immobilisation. PHAs are a group of water insoluble biodegradable biopolymers produced by a number of bacteria. Among PHAs, polyhydroxybutyrate (PHB) is a comercionally available short-chain-length polymer, which can be used for the immobilisation of a wide range of different enzymes by hydrophobic interactions in an easy way, without necessity of chemical crosslinking. In this study we have developed a cheap, simple, and effective method of trypsin immobilisation on the PHB submicron fiber membranes. The prepared biocatalyst has very good activity and stability after repeated applications. A laboratory model of a continuous enzyme reactors was equipped with the submicron fiber membranes containing immobilized trypsin and tested for continuous bovine β-lactoglobulin hydrolysis. The enzyme nano-membrane reactor with immobilized trypsin can be scaled up to produce hypoallergenic milk formulas, protein hydrolysates or biologically active peptides.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

<a href="/en/project/TH03020466" target="_blank" >TH03020466: Innovated equipment and technology for industrial nanofiber production by solution blow spinning.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů