Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027073%3A_____%2F10%3A%230000838" target="_blank" >RIV/00027073:_____/10:#0000838 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)
Original language description
The objective of the work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovická blata population in order to initiate in vitro cultures from them. The plastid DNAanalysis was used as a molecular-genetic tool. The trnL-trnF region of chloroplast DNA was tested by PCR-RFLP method; furthermore, the chloroplast microsatellite region Pt41093 was also analysed. All the tested individuals from the studied population matched the Pinus uncinata subsp. uliginosa haplotype. In bog pine, regeneration and development of axillary shoots were significantly enhanced in the presence of 5 g/L activated charcoal.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GK - Forestry
OECD FORD branch
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Project
<a href="/en/project/SP%2F2D4%2F83%2F07" target="_blank" >SP/2D4/83/07: Genetic diversity protection of the Czech subendemic pine (Pinus uncinata subsp. uliginova) using a combined method of biomonitoring, controlled pollination and micropropagation in the geographic centre of its distribution.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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