Aneuploidy detection in porcine embryos using fluorescence in situ hybridization

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F01%3A80000040" target="_blank" >RIV/00027162:_____/01:80000040 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Aneuploidy detection in porcine embryos using fluorescence in situ hybridization
Original language description
Very few data on the incidence and risk factors for aneuploidy, as a significant cause of reproductive disorders in farm animals, are available. Most aneuploid conceptuses die in utero and methods for early assessnebt of aneuploidy are needed. Porcine embryos were collected, transferred onto poly-L lysine-coated slides and fixed with 0.01 N HCl, 0.1 % Tween 20. A method of multi-color fluorescence in situ hybridisation was developed for detecting chromosomes 1, 10 and Y in blastomeres. Only embryos withextra signals were regarded as pathological. Eighty-five of the 93 examined embryos (91.4%) were diploid, 2 (2.6%) were tripolid, 5 (5.4%) were mosaic, 1 (1.1%) showed trisomy, and 44 (47.3%) showed 1 chromosome Y signal.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical
Chromosome research (14th International Chromosome Conference)
ISSN
0967-3849
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
suppl.1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
2
Pages from-to
77-78
UT code for WoS article
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EID of the result in the Scopus database
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