Cloning and expression of the haloalkane dehalogenase gene dhmA from Mycobacterium avium N85 and preliminary characterization of DhmA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F02%3A90000039" target="_blank" >RIV/00027162:_____/02:90000039 - isvavai.cz</a>

Alternative codes found

RIV/00027014:_____/03:00006688

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cloning and expression of the haloalkane dehalogenase gene dhmA from Mycobacterium avium N85 and preliminary characterization of DhmA

Original language description

Identification of the putative haloalkane dehalogenase gene dhmA from Mycobacterium avium subsp. avium, cloning of the gene and expresssion of recombinant DhmA in Escherichia coli was described. Sequencing and phylogenetic analysis of the sequence of dhmA was done and the putative catalytic triad and putative oxyanion hole of DhmA in catalytic site were proposed. Purification of wild type and the recombinant DhmA, and preliminary biochemical characterization was presented, and the function of M. avium DhmA in protection of the mycobacterial cells against halogenated substances was discussed.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied and Environmental Microbiology

ISSN

0099-2240

e-ISSN

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Volume of the periodical

68

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

3724-3730

UT code for WoS article

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EID of the result in the Scopus database

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