Assignment of chromosome rearrangements between X chromosomes of human and cattle by laser microdissection and Zoo-FISH

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F05%3A06000043" target="_blank" >RIV/00027162:_____/05:06000043 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Assignment of chromosome rearrangements between X chromosomes of human and cattle by laser microdissection and Zoo-FISH

Original language description

Cross-species fluorescence in situ hybridization was performed on cattle metaphase spreads using Homo sapiens X chromosome (HSAX) painting probes specific for the p- and q-arms to identify the cytogenetic location of a chromosome breakpoint between HSAXand the Bos taurus X chromosome (BTAX). Hybridization probes were generated by microdissection of HSAX p- and q-arms, amplification of the isolated chromosome material by DOP-PCR, and labelling of the PCR products with digoxigenin in a secondary PCR.Independent Zoo-FISH of the two painting probes on bovine metaphase chromosomes (detected by antidigoxigenin-fluorescein) resulted in clear hybridization signals on BTAX. A breakpoint was identified between HSAXp and HSAXq on BTAX, and narrowed downbetween the G-bands BTAXq25 and BTAXq26. The assumed centromere transposition between HSAX and BTAX associated with the rearranged chromosome segments is supported by cytogenetic assignments of the genes BGN and G6PD to BTAX.

Czech name

Stanovení chromozomálních přestaveb mezi X chromozomem člověka a skotu pomocí laserové mikrodisekce a Zoo-FISH

Czech description

Použití "arm-specific" DNA sond identifikovalo evoluční konzervaci mezi lidským p ramenem chromozomu X a distální částí q ramene bovinního chromozomu X. Lidské q rameno chromozomu X má částečnou homologii mezi bovinním Xp a proximální částí Xq. Tentocytogeneticky identifikovaný bod zlomu X chromozomu přináší nové informace o chromozomálních mutacích v genomové evoluci pohlavních chromozomů skotu a člověka.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chromosome Research

ISSN

0967-3849

e-ISSN

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Volume of the periodical

13

Issue of the periodical within the volume

6

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

569-574

UT code for WoS article

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EID of the result in the Scopus database

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