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Gilts and sows produce similar rate of diploid oocytes in vitro whereas the incidence of aneuploidy differs significantly

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F07%3A%230000240" target="_blank" >RIV/00027162:_____/07:#0000240 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Gilts and sows produce similar rate of diploid oocytes in vitro whereas the incidence of aneuploidy differs significantly

  • Original language description

    Oocytes derived from prepubertal gilts show reduced developmental competence when compared to oocytes collected from adult sows. Therefore, the aim of the study was to investigate whether gilts (4-5 months old) and adult sows (average age 3.5 years) of the same breed differ with regard to the rate of chromosomally unbalanced oocytes after IVM. COCs derived from individual pairs of slaughterhouse ovaries, were matured in vitro and analyzed cytogenetically by conventional staining (Giemsa) and FISH methods. Altogether, 72 females (31 sows, 41 gilts) and 430 secondary oocytes (194 and 236 oocytes of sows and gilts, respectively) were investigated. Cytogenetic analysis revealed diploid (Giemsa, FISH) and aneuploid (FISH) spreads. The incidence of diploid oocytes was similar for sows (26.0%) and gilts (24.5%) whereas the rate of aneuploid oocytes (nullisomic/disomic) was 8-times higher in gilts (10.8%) than in sows (1.3%). Diploid and aneuploid oocytes were observed in 64% of investigated f

  • Czech name

    Prepubertální prasničky a dospělé prasnice produkují stejné množství diploidních oocytů in vitro, ale incidence aneuploidií je vyšší u prasniček

  • Czech description

    Bylo zjištěno, že oocyty získané od prepubertálních prasniček mají sníženou vývojovou kompetenci a vyšší frekvenci aneuploidních oocytů oproti dospělým prasnicím.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GG - Zootechnics

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2007

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Theriogenology

  • ISSN

    0093-691X

  • e-ISSN

  • Volume of the periodical

    68

  • Issue of the periodical within the volume

    5

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    755-762

  • UT code for WoS article

  • EID of the result in the Scopus database