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Expression of ORF3 protein of porcine circovirus 2

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F08%3A%230000399" target="_blank" >RIV/00027162:_____/08:#0000399 - isvavai.cz</a>

  • Alternative codes found

    RIV/62157124:16170/08:00001817 RIV/62157124:16170/08:43871750

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Expression of ORF3 protein of porcine circovirus 2

  • Original language description

    The aim of this work was to express recombinant ORF 3 protein in a bacterial expression system and to demonstrate that infected animals produce ORF3 specific antibodies. We have successfully cloned ORF3 gene into expression vector pDest 17 and expressedrecombinant protein with molecular mass 15 kDa. ORF3 protein was deposited in bacterial cells in the form of inclusion bodies and had to be solubilised in 8M urea before purification. Although the concentration of ORF3 specific antibodies was considerably lower than the concentration of ORF2 antibodies, these antibodies were found in naturally infected as well as in vaccinated animals.

  • Czech name

    Exprese ORF3 proteinu prasečího cirkoviru 2

  • Czech description

    Cílem této práce bylo prostřednictvím bakteriálního systému vyprodukovat rekombinantní ORF 3 protein a otestovat, zda cirkovirem infikovaná zvířata produkují ORF 3-specifické protilátky. Podařilo se úspěšně naklonovat ORF 3 gen ve vektoru pDest 17 a exprimovat rekombinantní protein o molekulové hmotnosti 15 kDa. ORF 3 protein byl vložen do bakteriální buňky ve formě inkluzních tělísek a musel být pře vlastní purifikací rozpuštěn v 8M močovině. ORF 3-specifické protilátky byly detekovány jak u zvířat přirozeně infikovaných cirkovirem, tak u zvířat vakcinovaných, ale jejich koncentrace byla nižší než hladina ORF2-specifických protilátek.

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    GJ - Diseases and animal vermin, veterinary medicine

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA524%2F06%2F1640" target="_blank" >GA524/06/1640: The control of viremia by neutralizing antibodies and localization of neutralization epitopes of porcine circovirus 2</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    20th International Pig Veterinary Society Congress

  • ISBN

    978-0-620-41364-0

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

  • Publisher name

    Hein Jonker Media Management

  • Place of publication

    Durban, South Africa

  • Event location

    Durban, South Africa

  • Event date

    Jun 22, 2008

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article