Different evolutionary trails in the related genomes Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) uncovered by orthologous satellite DNA repositioning
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F08%3A%230000423" target="_blank" >RIV/00027162:_____/08:#0000423 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Different evolutionary trails in the related genomes Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) uncovered by orthologous satellite DNA repositioning
Original language description
Centromeric repetitive sequences from chromosome 4 (CCR4/10sat) of Cricetus cricetus (CCR, Cricetidae, Rodentia) were isolated using the laser microdissection, followed by DOP-PCR amplification and labelling. The probe was mapped by fluorescent in situ hybridisation onto C. cricetus and another member of Cricetidae, Peromyscus eremicus. The centromeric sequences showed to be present in another C. cricetus chromosome (CCR10) besides CCR4. Moreover, these almost chromosome-specific sequences revealed to be present in the P. eremicus genome, and most interestingly, displaying a ubiquitous scattered distribution throughout this karyotype. In both species, a co-localisation of CCR4/10sat with constitutive heterochromatin was found. The presence of these orthologous sequences in both genomes is suggestive of a phylogenetic proximity.
Czech name
Různé cesty evoluce u příbuzných druhů Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) stanovené přesunem orthologní satelitní DNA
Czech description
Centromerické repetitivní sekvence z chromozomu 4 (CCR4/10sat) Cricetus cricetus (CCR, Cricetidae, Rodentia) byly izolovány laserovou mikrodisekcí, amplifikovány pomocí DOP-PCR a značeny. Sonda byla mapována fluorescenční in situ hybridizací na chromozomy C. cricetus a dalšího zástupce čeledi Cricetidae, Peromyscus eremicus. Centromerické sekvence byly kromě CCR4 přítomny i na dalším chromozomu (CCR10) C. cricetus. U P. eremicus sonda vytvářela rozptýlený signálna všech chromozomech. U obou druhů byla nalezena společná lokalizace repetitivní CCR4/10sat s konstitutivním heterochromatinem. Přítomnost této orthologní sekvence u obou genomů naznačuje jejich fylogentickou příbuznost.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Micron
ISSN
0968-4328
e-ISSN
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Volume of the periodical
39
Issue of the periodical within the volume
8
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
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UT code for WoS article
000260873600010
EID of the result in the Scopus database
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