Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000558" target="_blank" >RIV/00027162:_____/09:#0000558 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro

Original language description

The aim of this study was to determine whether apoptosis of lymphocytes is modulated by muramyl dipeptide (MDP) that is a minimal structural unit of peptidoglycan in Gram-positive bacteria. To this aim, apoptosis of lymphocytes was studied in in vitro environment (RPMI medium, 37 °C, 5% CO2) with 10 ug.ml-1 MDP. Heifers were used as mammary gland cell donors for in vitro studies. Lymphocytes from the intact mammary glands were harvested following the phosphate buffered saline intramammary injection. Apoptosis and necrosis of lymphocytes were analysed by flow cytometry following simultaneous staining with Annexin-V and propidium iodide. The results of this study demonstrate that apoptosis of lymphocytes was delayed during in vitro incubation with MDP.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Slovak Journal of Animal Science

ISSN

1337-9984

e-ISSN

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Volume of the periodical

42

Issue of the periodical within the volume

Suppl.1

Country of publishing house

SK - SLOVAKIA

Number of pages

4

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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