Changes in functional parameters of cryopreserved boar spermatozoa capacitated in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000566" target="_blank" >RIV/00027162:_____/09:#0000566 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Changes in functional parameters of cryopreserved boar spermatozoa capacitated in vitro

Original language description

The aim of this study was to assess functional status of spermatozoa after cryopreservation, separation and capacitation to characterize the influence of these procedures on spermatozoa functional parameters. Boar semen were frozen-thawed, motile spermatozoa were separated on Percoll-gradient and capacitated by 1mM caffeine. Motility, viability and chromatine integrity of spermatozoa were evaluated by phase contrast, Annexin V-FICT apoptosis kit and SCSA assay respectively. The high cryotolerant- (HCT)and the low cryotolerant- (LCT) boars were classified in previous experiments. There were no differences in motility and viability spermatozoa between both boar groups before freezing but after thawing, the significantly higher motility and viability were found for HCT- compared with those for LCT-boars. Separation was more effective in the LCT- than in HCT- boars.. In both groups, DNA of spermatozoa was highly stable during cryopreservation, separation and capacitation.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GI - Farm animal breeding and farm animal pedigree breeding

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Research in Pig Breeding

ISSN

1802-7547

e-ISSN

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Volume of the periodical

3

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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