Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F10%3A%230000611" target="_blank" >RIV/00027162:_____/10:#0000611 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12520/10:00011436
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods
Original language description
Cyprinid herpesvirus-3(CyHV-3) is a causative agent of the lethal disease in koi and common carp that can adversely affect common and koi carp breeds.At present, several different PCR tests are in use.In this study, we compared the sensitivity of the single-round and nested PCR assays by testing the panel of field tissue samples(106) of koi and common carp and by testing tissue samples with known amounts of CyHV-3 DNA. The sensitivity of all the PCR methods was similar within testing the spiked homogenate sample with a known amount of CyHV-3 DNA (ca.12 DNA copies), with the exception of Gray PCR (25 copies). Detection levels of the individual PCR methods in field samples varied.The most sensitive and suitable seems to be two-round Bercovier method, detection of 44,3% of positive samples in total.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Result continuities
Project
<a href="/en/project/QH71057" target="_blank" >QH71057: Monitoring of the koi herpes virus disease (KHVD) occurrence in breeds of common carp in CR and testing of susceptibility of chosen strains of common carp to KHV.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of the World Aquaculture Society
ISSN
0893-8849
e-ISSN
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Volume of the periodical
41
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
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UT code for WoS article
000282697900011
EID of the result in the Scopus database
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