Distribution of Mycobacterium avium subsp avium and M. a. hominissuis in artificially infected pigs studied by culture and 1S901 and IS1245 quantitative real time PCR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F10%3A%230000638" target="_blank" >RIV/00027162:_____/10:#0000638 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Distribution of Mycobacterium avium subsp avium and M. a. hominissuis in artificially infected pigs studied by culture and 1S901 and IS1245 quantitative real time PCR
Original language description
Mycobacterium avium subsp. avium (MAA) and M. a. hominissuis (MAH) are frequently associated with diseases in animals and humans. The aim of this study was to develop a system for rapid and accurate real time quantitative PCR (qPCR) identification and quantification of MAA and MAH. This study included 22 per os infected pigs (ten were infected with MAA, ten with MAH and two negative controls). From each animal, 21 different tissue samples and blood were tested by microscopy, culture and qPCR. The developed triplex qPCR reaction was based on the simultaneous detection of specific insertion sequences, IS901 and IS1245, and also contained an internal amplification control. Contrary to culture examination, triplex qPCR confirmed the infection in all animals infected with MAA, and in eight animals infected with MAH. From the food safety point of view the presence of MAH in muscles should be considered as a possible threat to human health.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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